公司新闻/正文
kit-CreER工具鼠再登SCI--上海南方模式
人阅读 发布时间:2016-05-20 11:20
该研究利用谱系示踪技术揭示心脏c-Kit+干细胞在心脏生理稳态和损伤修复再生中主要形成冠状动脉内皮细胞,很少贡献心肌细胞,这为心脏干细胞及再生研究提供了理论基础和新的思路。研究成果于12月4日,在线发表于Cell Research(影响因子12.413)。
原文链接:http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2015143a.html
Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes
Abstract
Cardiac cells marked by c-Kit or Kit, dubbed cardiac stem cells (CSCs), are in clinical trials to investigate their ability to stimulate cardiac regeneration and repair. These studies were initially motivated by the purported cardiogenic activity of these cells. Recent lineage tracing studies using Kit promoter to drive expression of the inducible Cre recombinase showed that these CSCs had highly limited cardiogenic activity, inadequate to support efficient cardiac repair. Here we reassess the lineage tracing data by investigating the identity of cells immediately after Cre labeling. Our instant lineage tracing approach identifies Kit-expressing cardiomyocytes, which are labeled immediately after tamoxifen induction. In combination with long-term lineage tracing experiments, these data reveal that the large majority of long-term labeled cardiomyocytes are pre-existing Kit-expressing cardiomyocytes rather than cardiomyocytes formed de novo from CSCs. This study presents a new interpretation for the contribution of Kit+ cells to cardiomyocytes and shows that Kit genetic lineage tracing over-estimates the cardiogenic activity of Kit+ CSCs.